Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Yoshida, K.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Yoshida, K.
Agricola
Right arrow Articles by Yoshida, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1979, Vol. 20, No. 4 851-856
© 1979

Short communication

Interspecific and intraspecific mitochondria-induced cytoplasmic transformation in yeasts1

Kazuo Yoshida

Biological Institute, Faculty of Science, Nagoya University Nagoya 464, Japan

Spheroplasts from respiratory-deficient (rho–) yeasts were transformed into rho+ cells by incubation with mitochondria prepared from respiratory-sufficient (rho+) yeasts in the presence of polyethylene glycol 4000 and CaCl2. Spheroplasts of budding yeast rho– strains were also transformed into rho+ cells by treatment with mitochondria prepared from heterospecific budding or fission yeast strains. All the transformed regeneratants yielded rho+ yeast colonies which bore chromosomal genetic characteristics of the spheroplasts used and cytoplasmic genetic characteristics of the mitochondria used. These indicate that mitochondrial genes or mitochondria themselves introduced by the incubation functioned normally in the rho– cells regardless of the species difference of the recipient spheroplasts.

1A preliminary report appeared in ref. 17.

(Received October 31, 1978; )
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.