Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Koshiba, T.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Koshiba, T.
Agricola
Right arrow Articles by Koshiba, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1979, Vol. 20, No. 4 803-809
© 1979

Article

Alicyclic acid metabolism in plants 12. Partial purification and some properties of shikimate kinase from Phaseolus mungo seedlings

Tomokazu Koshiba

Department of Biology, Tokyo Metropolitan University Setagaya-ku, Tokyo 158, Japan

Shikimate kinase from Phaseolus mungo seedlings was partially purified by DEAEcellulose, hydroxyapatite and Sephacryl S-200 column chromatographies. The activity was completely inhibited by EDTA and the requirement for Mg2+ could be partially replaced by Mn2+, Ca2+; Co2+ and Cd2+. Sulfhydryl inhibitor did not inhibit the enzyme activity. The apparent Km values for shikimic acid and ATP at pH 8.6 were 0.25 mM and 0.38 mM, respectively. The activity appeared to be maximal at pH 8.6–9.0. Shikimate-3-phosphate and ADP inhibited the activity slightly. Aromatic amino acids, quinic acid and dehydroquinic acid had no significant effect on the activity.

(Received January 11, 1979; )
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.