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Plant and Cell Physiology, 1979, Vol. 20, No. 4 741-746
© 1979

Article

Thiamine requirement of two different cultured cell lines of soybean

Makoto Ikeda1, Kunihiko Ojima and Koji Ohira

Department of Agricultural Chemistry, Tohoku University Sendai 980, Japan

When supplied with 6-benzyladenine (0.5–5 mg/liter) instead of thiamine, thiamine-requiring soybean cells (strain TU) could grow successively. The effect of cytokinin was much more remarkable at a relatively higher concentration of 2,4-dichlorophenoxyacetic acid (4 mg/liter) than at a lower concentration (0.5 mg/liter). Among calli initiated from soybean hypocotyls on a medium without thiamine, the thiamine-nonrequiring variant (strain G) was obtained incidentally. As this cell line became green in light, it could be visually separated from the other necrotic tissues. Strain G cells could grow successively not only without thiamine but also without phytohormones, auxin and cytokinin. This cell line had relatively higher amounts of chlorophyll and thiamine, and grew in rigid, large cell aggregates which differed from cell aggregates of the strain TU cell line. The thiamine requirement of plant cultured cells seems to be associated with the degree of dedifferentiation of the cells rather than the kind of plant. In general, the higher the degree of redifferentiation of the cells, the higher is their thiamine level and the less they require externally supplied thiamine.

1Present address: Section of Phytochemical Research, Eisai Co., Ltd., Kawashima, Gifu 483, Japan.

(Received December 15, 1978; )
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