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Plant and Cell Physiology, 1979, Vol. 20, No. 4 733-739
© 1979

Article

Habituation in suspension-cultured soybean cells to thiamine and its precursors

Makoto Ikeda1, Kunihiko Ojima and Koji Ohira

Department of Agricultural Chemistry, Tohoku University Sendai 980, Japan

When thiamine concentration in subculture medium was rapidly lowered to nil, soybean cells in suspension became necrotic and stopped growing entirely. When it was gradually lowered, cell growth was vigorous until the concentration was reduced to 7.8×10–3 mg/liter. The cells at this level of thiamine ceased growing for a time, but prolonged culture in the same medium resulted in the appearance of fresh white cells which could be easily distinguished from the old brown, necrotic cells in the aggregates. These new cell lines could be subcultured with further reduction in the thiamine supply, growing as larger aggregates of about 4 mm in diameter.

New cell lines were similarly obtained by prolonged cultures in media containing a thiamine precursor; three lines appeared to be habituated to the pyrimidine moiety and one to the thiazole moiety. The latter cell line could be subcultured without thiamine and its precursors for at least eight passages. These habituated cells were characterized by the increase of the dry to fresh weight ratio and by their growth in large aggregates.

1Present address: Section of Phytochemical Research, Eisai Co., Ltd., Kawashima, Gifu 483, Japan.

(Received December 15, 1978; )
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