Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Mallery, C. H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Mallery, C. H.
Agricola
Right arrow Articles by Mallery, C. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1979, Vol. 20, No. 4 689-696
© 1979

Article

Developmental profile of intracellular compatments during Allium cepa root cell germination

Charles H. Mallery

Laboratory for Quantitative Biology, Department of Biology, University of Miami Coral Gables, FL 33124, U. S. A.

The efflux kinetics of 86Rb from 24 hr preloaded Allium cepa cv. Evergreen Long White Bunching root tip cells through the first 120 hr of germination have been documented. Estimates of the percent of isotope, the calculated amount present and the halftime of efflux of the three root cell compartments present were made. Of the total isotope present the % in the vacuole decreased from maxima of 64% to 40% with germination time, while the % of the isotope in the cytoplasm remained uniform near 39%. The free space increased maximally throughout germination to 20% by 120 hr. Rb content, based on specific activity calculations, of the slowest, intermediate and fastest compartments was seen to rise from low levels at 52 hr germination toward maximal values by 96 hr. Speculation as to when a germinating root becomes functionally mature was attempted by comparing the times of onset of major macromolecule synthesis (DNA, RNA, protein) to that of maximal Rb content. Stable mitotic and DNA synthetic indices occurred at the 10 mm (72 hr) stage with the initiation of major macromolecules syntheses occurring prior to 2 mm (40 hr). 86Rb efflux data suggests that the compartments do not contain maximal amounts until the 22 mm (96 hr) stage.

(Received December 26, 1978; )
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.