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Plant and Cell Physiology, 1979, Vol. 20, No. 1 201-212
© 1979

Article

Autolysis of the cell wall ß-D-glucan in corn coleoptiles

Donald J. Huber and Donald J. Nevins

Department of Botany and Plant Pathology, Iowa State University Ames, Iowa 50011, U.S.A.

Corn coleoptile cell walls prepared and incubated in buffer autolyzed as much as 100 µg per mg dry weight over a 36 hr period. This activity was attributed to the release of ß-D-glucan which constitutes as much as 110 µg per mg of the cell wall on a dry weight basis. Gel exclusion chromatography (Bio-gel P-2) of the autolytically solubilized products revealed the presence of a polymeric component and a monosaccharide, and time course studies showed that the polymeric component was progressively converted to monosaccharide. Glucose was the only monosaccharide detected. Treatment of the polymeric component with a bacterial glucanase specific for ß(1->3):ß(1->4) mixed-linkage glucans yielded distinctive tetra- and trisaccharides which is consistent with the hypothesis that it was derived from wall ß-D-glucan. At least 90% of the autolysis products were derived from this wall component.

The tolerance of autolytic activity to detergents and high salt concentrations provided evidence that the enzymes responsible are strongly associated with the wall.

(Received October 3, 1978; )
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