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Plant and Cell Physiology, 1961, Vol. 2, No. 1 9-24
© 1961

Article

ROLE OF SULFUR IN THE CELL DIVISION OF CHLORELLA, WITH SPECIAL REFERENCE TO THE SULFUR COMPOUNDS APPEARING DURING THE PROCESS OF CELL DIVISION II.

EIJI HASE, SAYOKO MIHARA and HIROSHI TAMIYA

Institute of Applied Microbiology, University of Tokyo, and Tokugawa Institute for Biological Research Tokyo

1. Chlorella cells, which had been grown synchronously under sulfur-deficient conditions and thus rendered unable to perform cell division, were made capable of nuclear and cellular division by being supplied with 35S-labeled sulfate and nitrate under photosynthesizing conditions, and the fate of sulfur during these recovery processes was followed.

2. When the S-starved cells were provided with sulfate alone under photosynthesizing conditions, cells grew appreciably in mass performing nuclear division but remaining incapable of cellular division. During these processes most of the 35S was found to be incorporated into the protein fraction of algal cells.

3. When the cells which had been stalemated at the above-mentioned stage were supplied with nitrate, they grew further in mass and eventually performed cellular division. During this period the 35S was found to be distributed not only in the protein fraction, but also in an appreciable amount in the cold and hot acid-soluble fractions.

4. By paper-electrophoretic experiments it was found that the nature of the sulfur substances appearing in the hot acid-soluble fraction changed strikingly during the process of cellular division. Zone electrophoresis and an anion-exchange chromatography of these substance isolated from the cells at the completion of cellular division, disclosed that they were most probably deoxypentose polynucleotides containing sulfur in some form yet unidentified.

5. It was demonstrated that there exist some antagonistic relations between the protein synthesis and the formation of these sulfur-containing deoxypentose polynucleotides, and that the former predominates under photosynthesizing conditions while the latter outweighs under nonphotosynthesizing conditions.

(Received August 9, 1960; )
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