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Plant and Cell Physiology, 1978, Vol. 19, No. 7 1271-1280
© 1978

Article

Intracellular hydroxyproline-rich glycoprotein of suspension-cultured tobacco cells

Hidetaka Hori and Tadashi Fujii

Institute of Biological Sciences, The University of Tsukuba Sakura-mura, Ibaraki 300-31, Japan

Two soluble glycoproteins containing hydroxyproline were extracted from cultured tobacco cells (cell line XD-6S) and purified by ion-exchange and gel-filtration chromatography. On DEAR-cellulose column chromatography in the final step of the purification, one was eluted at 90 mM NaCl and the other at 120 mM as single peak. Both purified glycoproteins were also sedimented as single peak with an ultracentrifugation. The S20,w values were 6.1 for the former and 7.0 for the latter.

These glycoproteins were composed of 94% polysaccharide and 6% protein in the former, and 87% polysaccharide and 13% protein in the latter. The sugar moiety consisted of galactose, arabinose, rhamnose, and uronic acid in both. Hydroxyproline accounted for 12% in the former and 20% in the latter amino acid composition. A high content of alanine in both (14 and 15%) was one of the distinctive characteristics of these soluble glycoproteins.

These intracellular soluble hydroxyproline-containing glycoproteins were not labelled within 30 min of incubation with 3H-proline, although the radioactivity was rapidly incorporated (within 15 min) into the intracellular macromolecules.

(Received February 21, 1978; )
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