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Plant and Cell Physiology, 1978, Vol. 19, No. 7 1185-1193
© 1978

Article

Wound-induced RNase in bean pod tissue II. Auxin regulation of RNase synthesis at transcription

Ann Varian and Joseph A. Sacher

Department of Biology, California State University Los Angeles, California 90032, USA

Both actinomycin D (ACTD) and auxin ({alpha}-naphthalene acetic acid) inhibit induction of bean pod RNase only if applied to tissue sections at the instant of cutting. Auxin inhibition varied from 25 to 75%. Studies of the kinetics of RNase degradation in the presence of cycloheximide showed that auxin has no effect on the half-time (t 0.5=2.7 hr) for degradation of RNase. This evidence and the temporal coincidence of inhibition of RNase induction by auxin and ACTD (only within the initial 15 to 30 min after wounding) indicate that auxin regulation is at transcription. Auxin also accelerates the natural decline in RNase 2.5-fold. The results are consistent with the hypothesis that auxin partially inhibits synthesis of the long-lived mRNA for RNase within the initial 15 to 30 min, resulting in a reduction in the amount of RNase synthesized. The enhancement of the natural decline in RNase by auxin is explicable in terms of auxin causing a reduction in the amount of RNase messenger, thus causing a perturbation of the ratio of mRNA for RNase to the regulator protein that would enhance blockage of RNase synthesis and thus accelerate the decline in RNase.

(Received March 27, 1978; )
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