Plant and Cell Physiology, 1978, Vol. 19, No. 4 715
© 1978
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Please replace the paragraph Culture of material in Material and method on page 574 (Fujii, Shimmen and Tazawa), Vol. 19, No. 4 with the following corrected paragraph.Materials and methods
Culture of material
Spirogyra sp. used for the experiments was collected in the river Kamogawa in Kyoto. Cylindrical cells composing the filament were 55 µm in diameter and 100200 µm in length. Each cell usually had one spiral ribbon-like chloroplast. The alga was cultured in slightly modified Reichardt's medium (27), 1000 ml of which contained: 200 mg KNO3, 20 mg K2HPO4, 10mg H3BO3, 6.6 mg FeSO4·7H2O, 25 mg Na2EDTA (ethylenediamine tetraacetic acid disodium salt), 200 mg NaHCO3, 50 mg CaSO4·2H2O, 10mg MgSO4·7H2O, 0.5 mg ZnSO4·7H2O, 5mg MnCl2·4H2O, 24 µg Na2MoO4, 2 µg CoCl2·6H2O, 500 mg Tris. The pH was adjusted to 7.4 with HCl. The alga was cultured in a Petri dish at 20±1°C under a 16 hr8 hr light-dark regime. The light intensity was about 2000 lux. Under such conditions, the cells divided once a day fairly synchronously.
Experimental solutions
Artificial pond water (APW) containing 0.1 mM each of KCl, NaCl and CaCl2