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Plant and Cell Physiology, 1978, Vol. 19, No. 4 685-689
© 1978

Article

Nitrate assimilation in Crotalaria juncea (Linn.) pollen suspension culture

Arvind Kapur, C. P. Malik and A. K. Dhawan

Department of Botany, Panjab Agricultural University Ludhiana, India

In vivo and in vitro activities of nitrate reductase were assayed in Crotalaria juncea pollen suspension cultures. This enzyme was found to be substrate-inducible and enhanced activity was observed when it was extracted with cysteine buffer or incubated with NADH (0.6 mM) at 25°C or when the germinated pollen grains were treated with red light for 10 min. Enzymes of ammonia assimilation, glutamate dehydrogenase and glutamate synthetase, and also the pentose phosphate-shunt enzyme, glucose-6-phosphate dehydrogenase, which catalyzes the step that provides reducing power to the system, are described.

(Received October 20, 1977; )
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