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Plant and Cell Physiology, 1978, Vol. 19, No. 4 609-616
© 1978

Article

RNA synthesis in the early stage of aerobic incubation of potato tuber discs

Takahide Sato, Akira Watanabe and Hidemasa Imaseki

Research Institute for Biochemical Regulation, Faculty of Agriculture, Nagoya University Chikusa, Nagoya 464, Japan

RNA synthesis of potato tuber discs during the early period of their aerobic incubation was investigated by feeding the discs with 3H-uridine. The rate of total RNA synthesis increased in two steps during the incubation. The increase during the first 2 to 3 hr was small, but that after 3 hr was large. The labeled RNAs were separated into poly(A) containing RNA [poly(A) (+) RNA] and poly(A) lacking RNA [poly(A) (–) RNA] by the use of a poly(U)-Sepharose column. Poly(A) (+) RNA was synthesized even in the freshly prepared discs which incorporated little 14C-leucine into a protein fraction, and the synthetic rate of poly(A) (+) RNA increased by about 50% during the first 3 hr incubation period, then gradually decreased thereafter. Synthesis of poly(A) (–) RNA continued to increase up to 7 hr after slicing. When the discs were pulse labeled, the proportion of radioactivity in poly(A) (+) RNA to that in the total RNA was maintained at about 50% until about 3 hr after slicing, but it abruptly decreased between 3 and 5 hr to about 35% which was maintained up to 9 hr after slicing.

(Received October 12, 1977; )
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