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Plant and Cell Physiology, 1978, Vol. 19, No. 4 561-571
© 1978

Article

Multiple effects of the inhibitory protein of ethylene production on cellular metabolisms1

Isao Todaka, Akira Watanabe and Hidemasa Imaseki

Research Institute for Biochemical Regulation, Faculty of Agriculture, Nagoya University Chikusa, Nagoya 464, Japan

The effects of an inhibitory protein of ethylene production isolated from etiolated mung bean hypocotyls (Planta 113: 115, 1973) were investigated. Etiolated mung bean hypocotyl segments incubated with IAA for 3 hr (1st incubation) to induce ethylene-producing activity were incubated for 1 hr with IAA in the presence of the inhibitory protein and a radioactive material to measure metabolic activity. Under the conditions where ethylene production was inhibited 80% or more by the protein, RNA synthesis, protein synthesis and phosphate uptake were suppressed 55–60, 65–80, and 60–75%, respectively. Conversion of 1-14C-acetate to CO2, lipid, basic and neutral fractions was also inhibited, but the degrees of inhibition were much less than those for the other processes. When the segments pretreated with the inhibitory protein during the 1st incubation period were washed free of the protein and assayed for their metabolic activities, the inhibition of RNA and protein syntheses and of phosphate uptake was partially restored, while ethylene-producing activity was fully restored to the control level. Similar reversible inhibitory effects were also observed for those metabolic activities in the tissue segments not treated with IAA, thus not producing induced ethylene. Oxygen uptake and conversion of U-14C-glucose to CO2 were not affected by the inhibitory protein. The possibility that the inhibitory protein acts on cell surface membranes and the modified membranes affect the regulatory mechanism of cellular metabolism is discussed.

1 This investigation was supported in part by grants from the Ministries of Education (B-248009), and of Agriculture and Forestry of Japan.

(Received November 4, 1977; )
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