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Plant and Cell Physiology, 1978, Vol. 19, No. 4 553-559
© 1978

Article

Effects of inhibitors on the enzyme system producing C6-aldehydes from C18-unsaturated fatty acids in chloroplasts of Japanese silver (Farfugium japonicum) leaves

Jiro Sekiya, Tadahiko Kajiwara and Akikazu Hatanaka

Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University Yamaguchi 753, Japan

When chloroplasts isolated from Farfugium japonicum (Japanese silver) leaves were used as an enzyme source, the activity of the enzyme system producing C6-aldehydes (cis-3-hexenal and n-hexanal) from C18-unsaturated fatty acids (linolenic and linoleic acids) decreased upon treatment with LAHase from potato. This enzyme system could not be separated from chlorophylls and lipids by detergent treatment and was not affected by light illumination, CCCP or DCMU. The activity of the enzyme system was inhibited by MB and NTB used as a redox reagent, SKF 525-A as an oxidase inhibitor and DABCO as a quencher of singlet oxygen, but not by DCIP, PMS and SOD. These data suggest that; i) interaction of the enzyme system with lipids is required for maximal enzyme activity, ii) this enzyme system may involve electron mediator(s), and iii) singlet oxygen takes part in the enzyme reaction.

(Received October 28, 1977; )
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