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Plant and Cell Physiology, 1978, Vol. 19, No. 3 491-500
© 1978

Article

Proteolytic enzymes in green wheat leaves I. Isolation on DEAE-cellulose of several proteinases with acid pH optima1

G. J. T. Frith, K. H. J. Gordon and M. J. Dalling

Plant Sciences Section, School of Agriculture and Forestry, University of Melbourne Parkville, Victoria, Australia 3052

Several peptide hydrolases have been isolated in a partially purified form from green wheat leaves by a combination of gel chromatography and chromatography on DEAE-cellulose. These hydrolases all degrade haemoglobin and other substrates tested at an acid pH. Three of the enzymes have been identified as proteinases by measurement of the total N: {alpha}-amino N ratio of the degradation products. These three enzymes have a similar optimum assay pH of about 4.5 with haemoglobin as substrate, but differ markedly in their stability at high temperatures.

1 Nomenclature in this paper follows the recommendation of the Commission on Biochemical Nomenclature (1972).

(Received November 30, 1977; )
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