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Plant and Cell Physiology, 1978, Vol. 19, No. 3 411-418
© 1978

Article

Nucleic acid contents and polyribosome formation in wheat embryos during germination and vernalization

Midori Tateyama1, Hiroko A. Ishikawa2 and Koh Ishikawa2

1 Department of Biochemistry, Faculty of Pharmaceutical Science, Higashi Nippon Gakuen University Ishikari-Tobetsu, Hokkaido 061-02, Japan
2 Department of Botany, Faculty of Science, Hokkaido University Sapporo 060, Japan

The contents of RNA and DNA in wheat embryo, determined by the STS method, were higher during cold treatment than the period of germination. The ratios of sRNA and IRNA to DNA and that of sRNA to total RNA for both periods were similar according to MAK column chromatography. The polyribosome contents were higher during cold treatment than germination. However, monosome contents were identical for both periods. During germination, the polyribosome content was the highest in embryos germinated for two days and continuously decreased thereafter. During cold treatment, polyribosome content was maximum in embryos cold-treated for 10 days then and decreased slowly for 60 days.

(Received August 12, 1977; )
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