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Plant and Cell Physiology, 1978, Vol. 19, No. 2 317-325
© 1978

Article

Effect of {alpha}-hydroxy-2-pyridinemethanesulfonate on glycolate metabolism in spinach leaf protoplasts1

Kazuaki Inoue, Mikio Nishimura and Takashi Akazawa

Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University Chikusa, Nagoya 464, Japan

The addition of {alpha}-hydroxy-2-pyridinemethanesulfonate ({alpha}-HPMS) to spinach leaf protoplasts caused a marked inhibition of photosynthetic CO2 fixation in both air and O2 atmospheres. In the O2 atmosphere, 14CO2 was incorporated into glycine, but upon addition of {alpha}-HPMS (10 mM), there was a suppression of 14CO2 incorporation into the glycine and serine plus isoleucine fractions, accompanied by an accumulation of 14C-glycolate. A marked stimulation of alanine labeling due to the {alpha}-HPMS treatment was also observed. Feeding protoplasts with [l–14C)-glycolate resulted in the formation of 14C-labeled glycine, serine, and sugar phosphates in both light and dark conditions, and a sizable amount of 14CO2 evolved concomitantly. The results support the notion concerning the operation of the glycolate pathway in leaf tissue during photorespiratory environments. The suitability of protoplasts for photosynthetic research in conjunction with the use of inhibitor substances are discussed, although the results of the present research indicate that the effect of a-HPMS is not confined to the specific inhibition of the glycolate-oxidase reaction.

1 This is paper 42 in the series "Structure and Function of Chloroplast Proteins", and the research was supported in part by the grant from the Ministry of Education of Japan (11912, 147106), the Toray Science Foundation (Tokyo), the Nissan Science Foundation (Tokyo), and the Matsunaga Foundation (to M. N.).

(Received July 21, 1977; )
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