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Plant and Cell Physiology, 1977, Vol. 18, No. 5 979-986
© 1977


Article

Three size classes of higher plant DNA-like RNA grouped by MAK column chromatography II. Species-specific grouping of DNA-like RNAs from various kinds of organisms

Takeshi Nitta1,1, Tsugio Shiroya2 and Ichiro Kaneko3

1 Laboratory of Radiation Biology, Faculty of Science, University of Tokyo Tokyo, Japan
2 Laboratory of Radiation Biology, Faculty of Science, University of Tokyo Tokyo, Japan
3 The Institute of Physical and Chemical Research Saitama, Japan

1 To whom all reprint requests should be sent. Present address: Department of Biology, Faculty of General Education, Tokyo University of Agriculture and Technology, Fuchu Tokyo 183, Japan

DNA-like RNAs from broad bean shoots, sea urchin embryos, T4-infected and non-infected Escherichia coli were fractionated by MAK column chromatography into three characteristic peaks. SDS-polyacrylamide gel electrophoresis and sucrose density gradient centrifugation of each RNA peak suggested that these peaks result from grouping of the RNAs by molecular weight. The three ranges for plant DNA-like RNA were different from those of phage-specific or bacterial mRNA. This may reflect some differences in the structures of DNA-like RNA between the organisms. Grouping of DNA-like RNA into three main size classes by an MAK column seems to be a general phenomenon occurring in a variety of organisms.

(Received February 21, 1977; )
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