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Plant and Cell Physiology, 1977, Vol. 18, No. 5 969-977
© 1977

Article

In vitro protein synthesis during germination and vernalization in winter wheat embryos

Shogo Fukushi, Koh Ishikawa and Kimiko Sasaki

Department of Botany, Faculty of Science, Hokkaido University Sapporo, Japan

Protein synthesis during germination at 24°C and vernalization at 4°C in winter wheat embryos were investigated with a cell-free system. During germination, the capacity for protein synthesis increased in the early stage between 12 and 36 hr of imbibition then declined to a final low level between 48 and 72 hr. This transition was due to quantitative changes of the activities of ribosomal and supernatant fractions in the early stage and mainly to those of the supernatant fraction in the later stage. During vernalization, the capacity for protein synthesis continued to decline over 15 to 60 days at 4°C. This transition was due to the change in activity of the supernatant fraction; the activity of the ribosomal fraction was nearly constant.

Electrophoretic analysis of in vitro products indicated that the high molecular weight proteins present in 12-hr embryos had disappeared in 48-hr germinated wheat embryos and that the products in 24- and 36-hr embryos were types intermediate between those of 12- and 48-hr embryos. The products in each vernalized embryo resembled those in 24- and 36-hr germinated embryos. Therefore, it was concluded that the mRNA species for translation changed during germination and vernalization in winter wheat embryos.

(Received January 20, 1977; )
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