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Plant and Cell Physiology, 1977, Vol. 18, No. 5 1159-1162
© 1977

Short Communication

The selective extraction of RNA polymerase I (A) from cauliflower inflorescence

Hirosuke Fukasawa and Tsuneo Uchiyama

Department of Biology, Faculty of Science, Kobe University Kobe 657, Japan

DNA-dependent RNA polymerase I(A) in cauliflower inflorescence is exclusively solubilised when the tissue is homogenized with TGMED-buffer (50 mM Tris-HCl pH 8.0, 5 mM MgCl2 0.1 mM EDTA, 1 mM dithiothreitol, 25% glycerol) containing 0.6 M (NH4)2SO4 and 20% Polyclar-AT (polyvinypyrrolidone).

(Received April 19, 1977; )
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