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Plant and Cell Physiology, 1977, Vol. 18, No. 3 671-680
© 1977

Article

Mechanisms of the acido- and thermophily of Cyanidium caldarium Geitler III. Loss of these characteristics due to detergent treatment

Isao Enami and Ikujirô Fukuda

Department of Biology, Faculty of Science, Science University of Tokyo Kagurazaka, Shinjuku-ku, Tokyo 162, Japan

The photochemical reactions of Cyanidium cells treated with various concentrations of Triton X-100 or digitonin were examined. As the concentration of Triton X-100 was increased, the following 4 responses were observed: inhibition of endogenous O2 evolution (above 0.005%), stimulation of the p-BQ Hill reaction (above 0.01%), loss of thermophily (above 0.03%) and loss of acidophily (above 0.5%). In the presence of Triton X-100 (0.1% of final concentration), the p-BQ Hill reaction showed optimum activity at 30°C and was completely inactivated at temperatures over 45°C, though the optimum activity in the absence of Triton X-100 was 45°C. The pH activity curve, however, was unchanged by treatment with Triton X-100. The loss of heat tolerance caused by Triton X-100 was observed not only in the Hill reaction but also in the photosystem I reaction. The thermophily of Triton X-100-treated cells was completely recovered after washing with distilled water.

The acidophily of the alga was lost after digitonin (0.01% of final Concentration) treatment without any loss of thermophily or the inactivation of photosystem I and II reactions. The p-BQ Hill activity of digitonin-treated cells was optimum at pH 7 and completely lost in acid pH regions, while the temperature dependency was unchanged by this treatment. The irreversible loss of acid tolerance of the photosystem I and II activities due to digitonin was confirmed by various acid and alkaline treatments.

(Received November 20, 1976; )
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