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Plant and Cell Physiology, 1977, Vol. 18, No. 3 641-655
© 1977

Article

Cell cultures vs. whole plants for measuring phytotoxicity I. The establishment and growth of callus and suspension cultures; definition of factors affecting toxicity on calli

S. Zilkah and J. Gressel

Department of Plant Genetics, The Weizmann Institute of Science Rehovot, Israel

Callus tissue has been isolated from 11 species, of 8 which are ‘weeds’ from which callus isolation has not been previously described. Continuous suspension cultures of four species have been started. Growth conditions were optimized for high yield. Logarithmic phase and stationary phase cells react differently to some inhibitors. The larger the inoculum size, the greater the concentration of inhibitor that must be used to achieve equal inhibition. Hormonal constitution of the medium affects inhibition by ‘auxin’ type herbicides. Growth kinetics, delayed response and recovery must be considered in assessing phytotoxicity. Highly uniform and controlled procedures are required for interspecific comparisons of many compounds.

(Received January 7, 1977; )
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