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Plant and Cell Physiology, 1977, Vol. 18, No. 3 539-550
© 1977

Article

Linolenic acid binding by chloroplasts

Takeshi Okamoto1 and Sakae Katoh

Department of Pure and Applied Sciences, College of General Education, University of Tokyo Komaba, Meguro-ku, Tokyo, Japan

The binding of linolenic acid with chloroplasts was investigated with 14C-labelled linolenic acid. The effect of the fatty acid on the activity of the electron transport system was also studied.

The amount of linolenic acid bound to chloroplasts increased with increasing concentration of the fatty acid added in a manner suggesting a cooperative mode of binding. At the highest concentration of linolenic acid added (100 µM), the molar ratio of bound fatty acid to chlorophyll was four.

The bound fatty acid to chlorophyll ratios were inversely proportional to the amounts of chloroplasts added. The bound fatty acid was released by addition of bovine serum albumin or by washing of the chloroplasts. The mode of release during repeated washing indicates that binding of linolenic acid to chloroplast membrane occurred through partition of the fatty acid between the membrane and the aqueous medium.

Time courses and temperature dependency of the development of linolenic acid-induced inhibition of the Hill reaction were markedly different from those of the fatty acid binding. The inhibition was at least partially reversible. The results indicate that inactivation of electron transport is due to disorganization of the functional integrity of the membrane caused by penetration of the fatty acid molecules into the hydrophobic region of membrane.

1 Present address: Biological Laboratory, Nippon Medical School, Kosugi, Kawasaki, Japan.

(Received December 16, 1976; )
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