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Plant and Cell Physiology, 1977, Vol. 18, No. 3 527-537
© 1977

Article

Changes in nicotinamide nucleotide content and glucose metabolism in wheat embryos during vernalization

Hiroko A. Ishikawa, Koh Ishikawa, Midori Tateyama1, Namio Takizawa2 and Shoichiro Usami3

Department of Botany, Faculty of Science, Hokkaido University Sapporo, Japan

Contents of nicotinamide nucleotides, NAD, NADP, NADH2 and NADPH2, in wheat embryos were determined during normal germination and cold treatment. They increased markedly in the early stage of germination then decreased later. During cold treatment, these nucleotides increased initially as in normal germination, but did not decrease in the later stages.

The C6/C1 ratios during germination decreased gradually with aging, but after about the fourth day increased. This suggests that the pentose phosphate pathway operates actively. During cold treatment, the C6/C1 ratio decreased slightly with aging. This suggests that the EMP pathway operates predominantly in the glucose metabolism. Based on these results, the correlation between NADPH2 and glucose metabolism during cold treatment was discussed.

Embryos which had absorbed labelled glucose were fractionated into several chemical components. The radioisotope accumulated mainly in the sugar fraction, with small amounts in the organic acid, amino acid and nucleic acid fractions. Changes in the content of each component showed that sugar, organic acid and amino acid increased during cold treatment.

1 Present address: Department of Biochemistry, Faculty of Pharmaceutical Science, Higashi Nippon Gaguen University, Ishikari-Tobetsu, Hokkaido 061-02, Japan.

2 Present address: Hokkaido Forest Products Research Institute, Asahikawa 070, Japan.

3 Present address: Kakuto 534, Komae City, Tokyo 182, Japan.

(Received November 1, 1976; )
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