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Plant and Cell Physiology, 1977, Vol. 18, No. 2 463-467
© 1977

Short communication

Differences in peroxidase activity and isoenzymes in embryogenic ane non-embryogenic ‘Shamouti’ orange ovular callus lines1

J. Kochba, S. Lavee and P. Spiegel-Roy

Institute of Horticulture, Agricultural Research Organization, Volcani Center Bet-Dagan, Israel

Peroxidase activity was assayed and isoenzyme pattern was determined by starch gel electrophoresis in ovular calli of the ‘Shamputi’ orange. Callus from an embryogenic line showed a much higher peroxidase activity than callus from a non-embryogenic line. Within each callus line activity increased with age of stock callus and age of subcultured callus. Peak activity coincided with appearance of embryoids in culture. The isoenzyme pattern revealed one band, C4, typical of embryogenic callus.

1 Contribution from The Volcani Center, Agricultural Research Organization, P.O.B. 6, Bet-Dagan, Israel. Series 1976, No. 143-E

(Received April 9, 1976; )
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