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Plant and Cell Physiology, 1977, Vol. 18, No. 2 413-420
© 1977

Article

Chromatographic separation, of extracellular acid phosphatase of tobacco cells cultured under Pi-supplied and omitted conditions

Yaéko Ninomiya, Katsuji Ueki and Sitiro Sato

Department of Botany, University of Tokyo Hongo, Tokyo, Japan

An extracellular acid phosphatase preparation of tobacco XD-6 cells cultured in suspension was resolved into three fractions by sequential chromatography. Two of these were neutral pyrophosphatase with diesterase activity, having optimum pH at 6.8. The other is a nonspecific acid phosphatase having optimum pH at 5.8. The latter was concluded to be involved in the increase in extracellular activity upon Pi-depletion.

(Received August 31, 1976; )
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