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Plant and Cell Physiology, 1977, Vol. 18, No. 2 393-397
© 1977

Article

Improved analysis of citrate and aconitate in plant tissues

Daniel R. Nelson and Robert W. Rinne

USDA, ARS, Agronomy Department, University of Illinois at Urbana-Champaign Illinois 61801, U.S.A.

Experimental conditions for an improved method for the determination of citrate from plant tissue are described utilizing the reaction of acetic anhydride and pyridine with citrate. Protein is removed from a sample with perchloric acid and the perchlorate ion concentration and pH of the resultant sample solution are not factors in the pyridine reaction. Recovery of citrate from tissue after perchlorate treatment is comparable to present chemical methods and the presence of naturally occurring anions and cations does not interfere with the assay.

(Received August 19, 1976; )
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