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Plant and Cell Physiology, 1974, Vol. 15, No. 6 971-978
© 1974

Article

Oxidation-reduction reactions between electron transfer components and hydrogen peroxide in photosystem II of chloroplasts

Umeo Takahama, Hiroshi Inoué1 and Mitsuo Nishimura

Department of Biology, Faculty of Science, Kyushu University Fukuoka 812, Japan

The light-induced oxygen evolution, photoreduction of 2,6-dichlorophenol indophenol (DPIP) and carotenoid photobleaching induced by carbonyl cyanide m-chlorophenylhydrazone (CCCP) were investigated with spinach chloroplast fragments in the presence of H2O2. Oxygen evolution in the presence of H2O2 was not inhibited by CCCP and was only partially inhibited by 5 µM 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) which completely inhibited the Hill reaction with DPIP. The degree of inhibition by DCMU was decreased by a simultaneous addition of CCCP. Carotenoid photobleaching in the presence of CCCP was stimulated by H2O2. The CCCP-induced carotenoid photobleaching was completely inhibited by DCMU. However, it was only partially inhibited by DCMU in the presence of H2O2. These data indicate that H2O2 donates electrons at a site between the CCCP-sensitive site and the reaction center of photosystem II and is reduced at a site between the DCMU-blocked site and the reaction center of photosystem II.

1Present address: Department of Biology, Kyushu Dental College, Kitakyushu 803, Japan.

(Received June 20, 1974; )
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