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Plant and Cell Physiology, 1974, Vol. 15, No. 6 1099-1113
© 1974

Article

Analysis of photosystem II using particle II preparation III. Roles of cytochrome b559 with different redox potentials and plastocyanin in the photosynthetic electron transport system1

Hirosi Huzisige and Nozomu Takimoto

Department of Biology, Faculty of Science, Okayama University Okayama 700, Japan

1. Using the P-II preparation (photosystem II preparation), the functional sites for Cyt-b559 with different redox potentials and for plastocyanin in the non-cyclic electron transport chain of chloroplasts were investigated.

2. In the absence of plastocyanin, the P-II preparation showed no light-induced absorption changes in Cyt-b559. However, upon the addition of a sufficient amount of plastocyanin, remarkable photooxidation of this cytochrome was observed at room temperature.

3. Parallel measurements of the light-induced absorption changes in both Cyt-b559 and plastocyanin revealed a close relationship between them, and indicated that Cyt-b559 and plastocyanin are located in series on the main path of the electron transport chain involving two photoreactions in PS-II.

4. Difference spectra and action spectra for the light-induced absorption changes in Cyt-b559 and plastocyanin offered additional evidence in support of the above conclusion.

5. Analysis of the relationship between the activity of ferricyanide Hill reaction and the contents of Cyt-b559 of different redox potentials showed that both forms of Cyt-b559 (i.e. high- and low-potential forms) play important roles under physiological conditions, being on the main pathway of the electron transport chain connecting PS-II and PS-I.

6. To these findings it is possible to give reasonable explanations based on our scheme presented previously, which suggested that PS-II contains not one but two different photoreactions. This scheme is much more elaborately supported by this study which shows the functional sites for Cyt-b559 with two different potentials.

1This work has been supported by a Grant from the Ministry of Education (Grant No. 844004), which we gratefully acknowledge. Part of this report has been presented at the Gordon Research Conference on Regulatory Mechanisms in Photosynthesis, at Tilton, N.H., U.S.A., Aug. 13–17, 1973.

(Received June 20, 1974; )
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