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Plant and Cell Physiology, 1974, Vol. 15, No. 2 331-340
© 1974

Article

Isolation of membranes containing protease from the cotyledons of germinating pea seeds

Masatoshi Nakano1 and Tadashi Asahi

Laboratory of Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Nagoya University Nagoya, Japan

Membranes containing protease were isolated from the cotyledons of germinating pea seeds by differential and two successive sucrose density gradient centrifugations. Membranes were recovered in the microsomal fraction, but could be clearly separated from membranes carrying antimycin A-insensitive NADH-cytochrome c reductase EC 1.6.2.1 [EC] ), which seem to come from the endoplasmic reticulum. The density of protease-containing membranes was 1.135. Membranes contained a lower amount of phospholipid as compared with the endoplasmic reticulum. Electron microscopic observations also showed that the membranes were different from the smooth-surfaced endoplasmic reticulum.

1Present address: Department of Pathology, Aichi Medical University, Nagakute, Aichi, Japan.

(Received September 12, 1973; )
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