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Plant and Cell Physiology, 1973, Vol. 14, No. 6 1205-1208
© 1973


Short Communication

Increases in activities of membrane-bound hydrolases in pea cotyledons during germination

Masatoshi Nakano1 and Tadashi Asahi

Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University Nagoya 464, Japan

Membrane-bound proteinase and acid phosphatase activities, but not cytosol proteinase activity, in pea cotyledons increased after lag phases during germination. The activity hydrolyzing N-{alpha}-benzoyl-D,L-arginine P-nitroanilide in the membrane fraction increased rapidly in the imbibition stage. Whether the increases are due to de novo synthesis of the enzyme proteins was studied.

1 Present address: Department of Pathology, Aichi Medical University, Nagakute, Aichi, Japan.


(Received May 28, 1973; )
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