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Plant and Cell Physiology, 1973, Vol. 14, No. 6 1201-1204
© 1973

Short Communication

Survival of poplar callus at super-low temperatures after cold acclimation1

Akira Sakai and Yasutake Sugawara

The Institute of Low Temperature Science, Hokkaido University Sapporo, Japan

Callus derived from the cambial area of poplar twigs survived freezing down to –120°C or even the temperature of liquid nitrogen (–196°C), after cold acclimation. Exposure to cool preconditioning temperatures for a length of time before being acclimated at 0°C is essential for enhancing fully the callus hardiness.

1 Contribution No. 1248 from the Institute of Low Temperature Science.

(Received May 30, 1973; )
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