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Plant and Cell Physiology, 1973, Vol. 14, No. 6 1143-1150
© 1973

Article

Changes in nucleic acid metabolism in barley seedlings during vernalization

Nobuyuki Shiomi and Shiro Hori

Department of Applied Biology, Radiation Center of Osaka Prefecture Sakai, Osaka, Japan

Changes in nucleic acid metabolism of barley seedlings during vernalization were investigated using thymidine-3H and uridine-3H.

DNA content increased in the early germination stage from the 1st to 3rd week in vernalized seedlings. In unvernalized seedlings, the most rapid increase was found in the late germination stage. RNA content in the vernalized seedlings increased after 1 week and reached maximum level after 3 weeks of vernalization treatment. The unvernalized seedlings had a comparatively high content at 2 days' germination which then gradually increased.

Much thymidine-3H was incorporated into DNA and uridine-3H into RNA fractions in the seedlings during early vernalization. On the contrary, without vernalization, heavy incorporation of thymidine-3H was delayed during the late germination stage. Incorporation of uridine-3H showed a linear increase.

A more detailed distribution of thymidine-3H and uridine-3H in the nucleic acids was examined by methylated albumin-coated kieselguhr column chromatography. A considerable amount of the incorporated uridine-3H was found in the tenaciouslybound ribonucleic acid (TB-RNA) in the vernalized seedlings.

(Received January 18, 1973; )
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