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Plant and Cell Physiology, 1973, Vol. 14, No. 6 1113-1121
© 1973

Article

Studies on the nutrition of rice cell culture I. A simple, defined medium for rapid growth in suspension culture

Koji Ohira, Kunihiko Ojima and Akio Fujiwara

Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University Sendai, Japan

We investigated the nutrient requirements of rice in liquid culture and developed a revised medium of mineral salts, sucrose, thiamine and 2,4-dichlorophenoxyacetic acid. The following major nutrients at the indicated concentrations were beneficial: NO3-N (40 mM), NH4-N (5.0 mM), P (2.0 mM) and K (40 mM). Cobalt, iodine, pyridoxine, nicotinic acid and m-inositol were not essential and were excluded from the revised R-2 medium. Growth was better with ammonium and nitrate together than with nitrate as the sole nitrogen source. Cell growth in the R-2 medium was superior to that obtained in B5, Heller, Murashige-Skoog and White media.

(Received March 31, 1973; )
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