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Plant and Cell Physiology, 1973, Vol. 14, No. 5 941-951
© 1973

Article

Mineral nutrition and auxin-induced growth of Triticum coleoptile sections

H. G. Burström

Department of Plant Physiology Box, 22007 Lund, Sweden

A revised method has been described for assaying auxin by the growth of Triticum coleoptile sections. With additions of Ca(NO3)2 10–4 and MgSO4 10–5 mol liter–1 the sensitivity and accuracy have been increased. This is mainly due to Ca. The coleoptiles obviously suffer from Ca-deficiency. The importance of a strict time schedule for manipulations is emphasiced. The duration of the tests is limited to 6 hr.Indole-3-acetic acid can be determined in concentrations down to 10–9mol liter–1; from 10–10 to 10–9mol liter–1 the log-log relation between concentration and growth is linear. Above 10–7 mol liter–1 elongation takes place under an abnormal increase in elastic extension, indicating that growth is limited by some unknown wallstabilizing factor. The interrelation between auxin, an anti-auxin and Ca are discussed.

(Received May 8, 1973; )
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