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Plant and Cell Physiology, 1973, Vol. 14, No. 5 865-872
© 1973

Article

Studies on the behavior of meiotic protoplasts II. Induction of a high fusion frequency in protoplasts from liliaceous plants

Michio Ito

Department of Biology, Faculty of Science, Nagoya University Nagoya 464, Japan

A method for the induction of high fusion frequencies in meiotic protoplasts of a lily and Trillium is described. Fusion was induced during the rapid isolation of protoplasts by quickly bringing about physical contact between protoplasts. Fused protoplasts were obtained in 50 to 90% yields from prophase cells and in 30 to 60% yields from cells in later phases. Large protoplasts with more than 10 nuclei were frequently produced. During culture, meiotic development was not synchronous in these protoplasts; each multinudeate maintaining a high degree of synchrony.

(Received February 28, 1973; )
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