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Plant and Cell Physiology, 1973, Vol. 14, No. 5 803-814
© 1973

Article

Ragi malt, a growth factor, for Vigna sinensis Endl. callus tissues1

K. B. S. Murthy Reddy2 and R. Narayana3

2Department of Botany, A.P.S. College Bangalore-19
3Department of Botany, University of Agricultural Sciences Bangalore-24, India

Callus tissue obtained from hypocotyl segments of Vigna sinensis was capable of indefinite growth on modified White's medium supplemented with 1 mg/liter each of 2,4-D and kenitin, but its rate of growth was slow. Ragi (Eleucine coracana L.) malt extract obtained from the ‘IE-810’ variety promoted a 38 and 30-fold increase in the fresh and dry weights, respectively, over the controls (modified White's with 2,4-D and kinetin). Similarly 100% ‘Aruna’ ragi malt extract elicited a nearly 40-fold increase in both the fresh and dry weights, which were as high as 21.1 g (fresh weight) and 1.64 g (dry weight) over the controls (0.48 for fresh and 0.04 g for dry weight). Growth values of 183 and 163, in respect to the fresh and dry weights were obtained in a period of 5 weeks.

Analysis of ‘Aruna’ ragi malt extract showed the presence of 17 amino acids. Totally 72% (Sugars 30, myo-inositol 10, amino acids 20 and inorganic fractions 12) of the growth promoting activity of ragi malt extract was accounted for. Ragi malt extract also exhibited cytokinin-like activity when assayed with Vigna callus which has an absolute requirement for a cytokinin and auxin.

1This work is a part of the dissertation of K. B. S. Murthy Reddy in fulfilment of the requirements for the degree of Doctor of Philosophy in Botany at the University of Mysore.

(Received March 28, 1973; )
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