Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hirai, M.
Right arrow Articles by Asahi, T.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Hirai, M.
Right arrow Articles by Asahi, T.
Agricola
Right arrow Articles by Hirai, M.
Right arrow Articles by Asahi, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1973, Vol. 14, No. 5 1019-1029
© 1973

Article

Membranes carrying acid hydrolases in pea seedling roots

Masashi Hirai and Tadashi Asahi

Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University Nagoya, Aichi 464, Japan

Subcellular localization of acid hydrolases in pea seedling roots was studied by differential and sucrose density gradient centrifugations. Significant parts of hydrolase activities in the tissue were recovered in mitochondrial and microsomal fractions. Sedimentable phosphatase was separated into two subtractions: denser and lighter membrane fractions. The distribution of phosphatase activity after sucrose density gradient centrifugation of the denser fraction coincided with that of antimycin Ainsensitive NADH-cytochrome c reductase activity. Electron microscopic observations suggested that the fraction contained only microsomes. RNase in the denser fraction seemed to associate with ribosomes. Phosphatase and RNase were solubilized by sonic treatment in the presence of high concentrations of salt. On the other hand, a-amylase was tightly bound to a membrane. The results are discussed with special regard to the relationship between the membranes and lysosomes.

(Received May 4, 1973; )
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.