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Plant and Cell Physiology, 1973, Vol. 14, No. 1 77-90
© 1973

Article

Anion-dependent changes of energy transfer in chloroplasts II. Relationships of the coupling factor to light-induced proton transport and absorbance change at 515 nm

Norikatsu Ikehara1 and Mitsuo Nishimura

Department of Biology, Faculty of Science, Kyushu University Fukuoka 812, Japan

Roles of the coupling factor in light-induced proton transport and 515-nm absorption change were investigated in chloroplasts washed with high concentrations of Tris salts (pH 7.2). Washing the chloroplasts with Tris-HCl and Tris-HNO3 buffers diminished both the light-induced pH rise and absorbance change at 515-nm, while Tris-H2SO4 buffer was much less effective. Inhibited activities could be restored by replacement of the coupling factor after extraction with EDTA. N,N'-dicyclohexylcarbodiimide also restored both activities. Effects of various anions on the proton pump and 515-nm shift were also investigated. The order of effectiveness was NO3–>Cl–>SO42–. The role of the coupling factor and its mode of action; the action mechanisms of Tris and anionsn energy transducing processes in chloroplasts, photophosphorylation, proton transport and absorbance change at 515 nm, are discussed.

1Present address: Biology Department, College of Science and Engineering, Ryukyu University, Naha, Okinawa, Japan.

(Received June 27, 1972; )
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