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Plant and Cell Physiology, 1973, Vol. 14, No. 1 51-60
© 1973

Article

Inhibition by adenine compounds of the induced formation of photosynthetic pigments in Rhodopseudomonas spheroides cells under dark-semiaerobic conditions

Masataka Higuchi

Laboratory for Radioisotopic Research, Tohoku University, School of Medicine Sendai 980, Japan

Effects of adenine, adenosine, AMP, ADP and ATP on the induced formation of bacteriochlorophyll and carotenoids in cell suspensions of dark-aerobically grown Rhodopseudomonas spheroides were examined under dark-semiaerobic conditions where no significant cell growth occurred. Pigment formation was strongly inhibited by 3 mM adenine, adenosine, AMP or ATP, but less strongly by ADP. Inhibition by either adenosine or ATP was completely reversible. Addition of 3 mM adenosine resulted in complete inhibition of pigment formation, while inhibition by more than 10 min ATP did not exceed 80%. No accumulation of any precursor-like pigments of either bacteriochlorophyll or carotenoids was observed in cells incubated in the presence of adenine compounds. Amounts of exogenously-added adenine, adenosine, or AMP decreased significantly during incubation, whereas the amount of exogeneously-added ATP or ADP did not appreciably decrease. Addition of 3 mM ATP or adenosine also significantly suppressed 3H-leucine incorporation into bacterial proteins. Nucleosides other than adenosine were ineffective in inhibiting the induced formation of photosynthetic pigments, indicating that the inhibitory action is specific to adenine compounds. It was assumed that both adenosine and ATP inhibit chromatophore formation rather than a particular step(s) in the biosynthetic pathways of the photosynthetic pigment, and that ATP exerts its effect from outside the cells, whereas adenosine does so after being taken up by the cells.

(Received July 24, 1972; )
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