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Plant and Cell Physiology, 1973, Vol. 14, No. 1 29-38
© 1973

Article

RNA synthesis in germinating red bean seeds

Akira Watanabe1, Takeshi Nitta and Tsugio Shiroya

Laboratory of Radiation Biology, Faculty of Science, University of Tokyo Hongo, Bunkyo-ku, Tokyo, Japan

Nucleic acids from 32P-labelled germinating red bean seeds were investigated by means of MAK column chromatography. 1) In cotyledons, synthesis of D-RNA occurred in the early stages of germination, 3 to 24 hr after the onset of imbibition. 32P was also incorporated into rRNA continuously at rather a moderate rate. DNA-RNA hybridization experiments revealed that the proportion of heterogeneous RNA (D-RNA) to rRNA decreased gradually. Nucleotide analysis suggested that tRNA was synthesized de novo, and that its CCA-end exchange was remarkable at early stages of germination. 2) In embryos, however, the incorporation of 32P into rRNA was very much greater than into D-RNA, and the exchange reaction at CCA-end of tRNA was not detected. The role of D-RNA, found in cotyledons in the initial stages of germination, was discussed.

1Present address: Research Institute for Biochemical Regulation, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya, Japan.

(Received May 10, 1972; )
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