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Plant and Cell Physiology, 1970, Vol. 11, No. 4 589-598
© 1970

Article

Effects of N-1-naphthylphthalamic acid on the growth and bud formation of tobacco callus grown in vitro

KUO AO FENG1 and A. J. LINCK

Department of Horticultural Science, University of Minnesota St. Paul, Minnesota, U. S. A

The effect of N-1 -naphthylphthalamic acid (NPA), indole-3-acetic acid (IAA) and kinetin on callus growth and bud formation was studied mainly by a tobacco callus culture method. Callus produced from Nicotiana tabacum var. Wisconsin 38 was used as the test plant material. Callus growth on nutrient agar containing 2 mg/liter of IAA was promoted by NPA added at a concentration of 0.5 mg/liter with 0.4 mg/liter of kinetin or by NPA added at 5 mg/liter in the absence of kinetin. At a high concentration of 50 mg/liter, however, NPA inhibited growth on the medium containing 2 mg/liter IAA and no kinetin. Kinetin reduced this NPA inhibition. In the presence of 0.4 mg/liter kinetin and 2 mg/liter IAA, when the concentration of NPA was 50 mg/liter, buds were initiated after calluses were grown on the test medium for 7 weeks in dim light, but no buds formed when NPA was omitted from the above medium.

The control of callus growth and bud initiation is based on the active ratio of auxin (IAA) to cytokinin (kinetin) in the medium and NPA added to the medium can promote or inhibit callus growth and induce bud formation. Therefore, it is proposed that NPA can itself reduce auxin activity or enhance cytokinin activity and hence change the active ratio of the two regulators. NPA may enhance the activity of cytokinin (here supplied as kinetin) but cannot substitute for it.

1Present address: Department of Biology, Wisconsin State University, Oshkosh, Wisconsin 54901, U. S. A.

(Received March 10, 1969; )
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