Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by SPLITTSTOESSER, W. E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by SPLITTSTOESSER, W. E.
Agricola
Right arrow Articles by SPLITTSTOESSER, W. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1970, Vol. 11, No. 4 579-588
© 1970

Article

Characterization of aspartate aminotransferase in germinating pumpkin cotyledon

WALTER E. SPLITTSTOESSER

Department of Horticulture, University of Illinois Urbana, Illinois 61801, U.S.A

During germination an increase in aspartate aminotransferase (E.C. 2.6.1.1 [EC] .) occurs in the cotyledons of pumpkin seedlings grown either in the light or dark with the bulk of the enzyme activity in the soluble fraction. The soluble and particulate enzymes had a pH optimum of 8.0, an {alpha}-ketoglutarate optimum of 0.02 mM and a broad aspartate optimum from 0.05 to 0.2 mM. The velocity of the reaction was proportional to the enzyme concentration over a wide range. The soluble enzyme was shown to require manganese and pyridoxal-5-phosphate and an active sulfhydryl group for maximum activitiy. The soluble enzyme was easily destroyed by pepsin while the particulate enzyme lost activity upon washing. It is suggested that aspartate aminotransferase plays a role in nitrogen metabolism during protein hydrolysis in germinating pumpkin cotyledons.

(Received January 6, 1970; )
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.