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Plant and Cell Physiology, 1970, Vol. 11, No. 4 569-578
© 1970

Article

Methionine-lysine-threonine-isoleucine interrelationships in the amino acid nutrition of rice callus tissue

KATSUHISA FURUHASHI and MICHIHIKO YATAZAWA

Department of Agricultural Chemistry, Faculty of Agriculture Nagoya University, Nagoya, Japan

Growth of rice callus tissue is discouraged when methionine is excluded from CMAA medium. While determining the methionine elimination effect, the amino acid interrelationships among methionine, lysine, threonine and isoleucine in the nutrition of the callus tissue were found. Poor growth, found in cultures on methionine deficient media was seen only when the media contained both threonine and lysine, simultaneously. The substitution of homoserine for methionine was also observed.

Determination of free amino acid composition in tissues revealed that free methionine was barely detectable in tissues grown with sufficient amounts of threonine and lysine. When the concentration of either threonine or lysine was reduced, the free methionine content of the tissue increased. When the methionine deficient medium was supplemented with homoserine, the free methionine in the tissue increased, although the tissue retained a considerable amount of free threonine and lysine. Cultivation of tissue on an isoleucine deficient medium resulted in a significant decrease in free threonine content.

These experimental results suggest that the biosynthetic pathway to methionine is cooperatively inhibited by threonine and lysine, and that threonine decomposition is inhibited by its end product isoleucine.

(Received February 19, 1970; )
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