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Plant and Cell Physiology, 1970, Vol. 11, No. 4 531-539
© 1970

Article

Modification of OGUR-ROSEN method for estimation of RNA in fresh and wounded sweet potato root tissues and change in RNA contents after the cutting1

YOSHIHIKO NAWA, TADASHI ASAHI and IKUZO URITANI

Laboratory of Biochemistry, Faculty of Agriculture Nagoya University, Chikusa, Nagoya, Japan

The suitability of the OGUR-ROSEN method for RNA extraction from sweet potato root tissue was investigated. The PCA extract from the freshly sliced tissue was colorless and showed a typical spectrum of RNA. However, as the slices were incubated for 1 to 3 days (wounded tissues), extract from them was brownish and revealed no typical spectrum of RNA. When wounded tissue was treated with acetone containing isoascorbic acid and the resultant acetone powder was treated with PCA, extract indicated the spectrum very close to that of RNA. Interfering substances still remaining were removed satisfactorily with charcoal which adsorbed RNA selectively. Thus, RNA could be assayed as the OD260-difference between PCA extract from the acetone powder and a fraction of the extract non-adsorbable on charcoal. Time course analysis of RNA contents during incubation of the tissue slices disclosed a remarkable increase in the total RNA in the initial stage of incubation.

1This paper constitutes part 81 of Phytopathological Chemistry of Sweet Potato with Black Rot and Injury

(Received September 5, 1969; )
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