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Plant and Cell Physiology, 1970, Vol. 11, No. 2 335-344
© 1970

Article

Separation and partial characterization of chloroplast and cytoplasmic ribosomes from Euglena gracilis1

LETICIA R. MENDIOLA, ANNA KOVACS and C. A. PRICE

Department of Biochemistry and Microbiology, Rutgers University New Brunswick, New Jersey 08903, U.S.A.

Chloroplast and cytoplasmic ribosomes from Euglena gracilis were separated by centrifugation in zonal rotors. The particles were characterized by their sedimentation rates as well as by their RNA components. Total extracts from green cells contained 30S, 55S and 89S particles or their aggregates, depending upon the Mg++ concentration. Extracts from fractions enriched for chloroplasts contained essentially 30S and 55S particles, while the supernatant (obtained after sedimentation of the chloroplasts) contained predominantly 89S particles or aggregates of cytoplasmic ribosomes. The 30S and 55S ribosomes contained RNA components which were unique and distinct from those of the cytoplasmic ribosomes. We were unable to detect 70S particles from the chloroplast preparations. Under our conditions, chloroplast extracts yielded 30S and 55S subunits or a series of rapidly sedimenting particles, possibly polysomes. Despite a variety of extraction techniques, we were unable to detect 70S particles from the chloroplasts.

1This study was supported in part by grant No. HD 01787 from the U. S. Public Health Service. Journal paper of the New Jersey Agricultural Experiment Station

(Received December 3, 1969; )
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