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Plant and Cell Physiology, 1970, Vol. 11, No. 2 315-321
© 1970

Article

Der Einfluß verschiedener Lichtqualitäten auf Chlorophyllgehalt und Wachstum von Gewebekulturen aus Crepis capillaris (L.) WALLR.

WOLFGANG HÜSEMANN

Pflanzenphysiologisches Institut der Freien Universität Berlin 1 Berlin 33, Königin-Luise Strße 1-3, Deutschland

The influence of different light qualities on chlorophyll content and growth of tissue cultures from Crepis capillaris (L.) WALLR.

Tissue cultures from Crepis capillaris growing on media (M1 ; M2 ; M2-E) formed chlorophyll and intact chloroplasts only in the short wave length region of the visible spectrum (350–550 nm). In red light (600–700 nm) as well as in darkness they lost their chlorophyll after 8–10 weeks.

The growth of Crepis-cultures was strongly influenced by light and the nitrogen of the medium. The highest increase in fresh weight (425–485% increase in 3 weeks) was attained in red light or in darkness on M2 by cultures which had lost their chlorophyll completely. M2 contains nitrates, ammonium salts and amino acids. In contrast, the increase in fresh weight of green cultures growing on M2 in blue or white light was considerably lower (155–180% increase in 3 weeks).

Omission of amino acids, (M2-E), resulted in the reduction of the growth (increase of fresh weight in 3 weeks: 120%) of the chlorophyll-free cells growing in the dark. Green cultures behaved differently on M2-E. In white light they attained an increase in fresh weight of 245%. This suggests that the growth promoting effect of the amino acids can be replaced by light.

Results with cultures growing on M1, which contains neither ammonium salts nor amino acids, point in the same direction. Green cultures in white or blue light grew better (90–100% increase in fresh weight in 3 weeks) on this "deficient" medium than chlorophyll-free tissues in red light or in darkness (20–30% increase in fresh weight in 3 weeks). Some aspects of these findings which concern the effect of light on growth are discussed.

(Received November 28, 1969; )
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