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Plant and Cell Physiology, 1970, Vol. 11, No. 2 221-229
© 1970


Article

Reductive carboxylation and amination of keto acids by spinach chloroplasts

AKIRA TSUKAMOTO

Department of Biology, Faculty of Science, Ochanomizu University Tokyo, Japan

1. In spinach chloroplasts, the occurrence of malic enzyme, isocitrate dehydrogenase, glutamate dehydrogenase and alanine dehydrogenase was confirmed.

2. In the presence of ammonia, pyruvate and {alpha}-ketoglutarate and pyruvate were photoreductively aminated to glutamate and alanine, respectively.

3. In the absence of ammonia, pyruvate and {alpha}-ketoglutarate were photoreductively carboxylated to malate and isocitrate, respectively.

4. Photoreductive carboxylation of pyruvate and {alpha}-ketoglutarate was suppressed by molecular oxygen. Inhibition was partly due to oxidation of photoreduced NADP or NAD.

(Received August 4, 1969; )
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