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Plant and Cell Physiology, 1969, Vol. 10, No. 2 411-423
© 1969

Article

Changes in respiratory metabolism in tissue cultures of carrot root

A. KOMAMINE, Y. MOROHASHI and M. SHIMOKORIYAMA

Department of Botany, Faculty of Science, University of Tokyo Hongo, Tokyo, Japan

Changes in respiratory metabolism accompanied by callus formation in cultured explants of carrot root were followed and the following results were obtained. 1) When the explant was cultured on a medium containing kinetin and 2,4-D, active cell division occurred and resulted in callus formation by the 9th–12th days. 2) Fresh weight remarkably increased after a lag-time of about 5 days. Changes in protein content on fresh weight basis were similar to changes in fresh weight. 3) Respiration rate increased during the first few days, when growth could not be distinctly measured. Accompanying the rise in respiration, the C6/C1 ratio also increased. As callus developed, the respiratory rate and C6/C1 ratio gradually decreased and RQ, became higher than unity. 4) Alcohol dehydrogenase activity increased between the 4th and 9th days after culture. 5) When sub-cultured callus tissue was fed with G-U-14C, some radioactivity was detected in the alcohol of the tissues. 6) These results suggest that during the first 4–6 days after culture the activity of the EMBDEN-MEYERHOF-PARNAS-TCA pathway was remarkably increased and, as callus developed, the relative participation of the pentose phosphate pathway gradually increased and simultaneously alcohol fermentation occurred.

(Received December 13, 1968; )
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