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Plant and Cell Physiology, 1969, Vol. 10, No. 2 337-348
© 1969

Article

The possibility of photo-induced induction of nitrate reductase in rice seedlings

MASARU SHIBATA1, MICHIHARU KOBAYASHI and EIICHI TAKAHASHI

Department of Agricultural Chemistry, Kyoto University Kyoto, Japan

Nitrate reductase activity in rice seedlings showed daily fluctuations. Seedlings placed in the dark slowly lost activity and quickly regained it when exposed to sunlight. Etiolated seedlings also showed rapid increases in activity when transferred to sunlight. This increase in activity by sunlight was inhibited by both chloramphenicol and ethionine. Ethionine inhibition was reversed by methionine. Purification of nitrate reductase was carried out by ammonium sulfate fractionation and DEAE-cellulose column chromatography. Nitrate reductase was purified about 40-fold from plants placed in the dark and in sunlight. Incorporation of mediionine-S-14CH3 into the nitrate reductase fraction was studied. In sunlight, the specific radioactivity of the nitrate reductase fraction from the DEAE-cellulose column increased 2-fold as compared with that of the crude extracts. Specific radioactivity did not increase in the dark.

1Present address: Asahi Kasei Chemicals Industry Co., Ltd., Sameshima 2-1, Fuji, Shizuoka, Japan

(Received December 3, 1968; )
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